Stand – Leica DMi8 automated microscope
Objectives – 10X NA 0.4, 20X NA 0.75, 25X NA 0.95 NA (water), 40X NA 1.4 (oil), 63X NA 1.4 (oil), 63X NA 1.2 (water)
Stage – Encoded, closed loop automated stage with autofocus and Piezo Z axis
Illumination – 80 MHz pulsed white light laser tunable in 1 nm increments from 440-790 nm with 8 lines available simultaneously; 405 nm continuous laser
Detector – 5 HyD detectors with spectrometer for spectral scanning from 410-850 nm
Confocal – Stellaris8 resonance scanning confocal scan head
Super-resolution – Lightning deconvolution based super-resolution to 150 nm
Time-correlated photon counting – Enables fluorescence lifetime measurements and fluorescence cross correlation spectroscopy
Computer – HP, Intel Xeon Gold w/ 8 cores, 192 GB RAM, NVIDIA GPU w/ 24 GB RAM
Software – LasX with Tau-sense, Phasors, Lightning, 3D Visualization
Primary Uses – Optical sectioning confocal, FLIM, FCCS, Super-resolution, FRAP, DIC
