Pollard Lab

Western Washington University

Publications

* Pollard Lab Undergraduate ** Pollard Lab Graduate

**Wienecke, A., *Barry, M. L., & Pollard, D. A. (2023). Natural variation in codon bias and mRNA folding strength interact synergistically to modify protein expression in Saccharomyces cerevisiaeGenetics224(4). https://doi.org/10.1093/genetics/iyad113 

Chou, H. T., Valencia, F. E., Alexander, J., Bell, A. D., Deb, D., Pollard, D. A., & Paaby, A. B. (2021). Diversification of small RNA pathways underlies germline RNAi incompetence in wild C. elegans strains. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2021.08.21.457212 

Lee, S. R., Pollard, D. A., Galati, D. F., Kelly, M. L., *Miller, B., Mong, C., Morris, M. N., Roberts-Nygren, K., Kapler, G. M., Zinkgraf, M. S., Dang, H. Q., Branham, E., Sasser, J., Tessier, E., Yoshiyama, C., Matsumoto, M., & *Turman, G. (2021). Disruption of a ∼23–24 nucleotide small RNA pathway elevates DNA damage responses in Tetrahymena thermophilaMolecular Biology of the Cell32(15), 1335–1346. https://doi.org/10.1091/mbc.e20-10-0631 

Pollard, D. A., Pollard, T. D., & Pollard, K. S. (2019). Empowering statistical methods for cellular and molecular biologists. Molecular Biology of the Cell30(12), 1359–1368. https://doi.org/10.1091/mbc.E15-02-0076

Kuo, S., Egertson, J., Merrihew, G., MacCoss, M., Pollard, D., & Rifkin, S. A. (2019). A simple mass-action model predicts protein timecourses from mRNA trajectories during a dynamic response in two strains of S. cerevisiae. bioRxiv, https://doi.org/10.1101/805846 

Turman G*, Pollard DA (2019). NucJuke: A tool for re-ranking gRNAs based on chromatin accessibility for CRISPR-Cas9 Experiments in yeast. http://nucjuke.biol.wwu.edu 

Pollard DA, Asamoto C*, Rahnamoun H*, Abendroth A*, Lee SR, Rifkin SA (2016). Natural Genetic Variation Modifies Gene Expression Dynamics at the Protein Level During Pheromone Response in Saccharomyces cerevisiae. BioRxiv.org. https://doi.org/10.1101/090480 

Pollard DA, Rockman MV (2013). Resistance to Germline RNAi in a Caenorhabditis elegans Wild Isolate Exhibits Complexity and Non-Additivity. G3, g3.113.005785. https://doi.org/10.1534/g3.113.005785 

Pollard DA (2012). “Design and Construction of Recombinant Inbred Lines” in Quantitative Trait Loci (QTL) : Methods and Protocols. Rifkin SA (Ed). Springer 1:871.

Li XY, MacArthur S, Bourgon R, Nix D, Pollard DA, Iyer VN, Hechmer A, Simirenko L, Stapleton M, Luengo Hendriks CL, Chu HC, Ogawa N, Inwood W, Sementchenko V, Beaton A, Weiszmann R, Celniker SE, Knowles DW, Gingeras T, Speed TP, Eisen MB, Biggin MD (2008). Transcription factors bind thousands of active and inactive regions in the Drosophila blastoderm. PLoS Biol. 2008 Feb;6(2):e27. https://doi.org/10.1371/journal.pbio.0060190 

Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Drosophila 12 Genomes Consortium (2007). Evolution of genes and genomes on the Drosophila phylogeny. Nature, 2007. 450(7167):203-18. https://doi.org/10.1038/nature06341 

Pollard DA, Iyer VN, Moses AM, Eisen MB (2006). Widespread Discordance of Gene Trees with Species Tree in Drosophila: Evidence for Incomplete Lineage Sorting. PLoS Genetics, 2006. 2:10. https://doi.org/10.1371/journal.pgen.0020173 

Moses AM, Pollard DA, Nix DA, Iyer VN, Li X, Biggin MD, Eisen MB (2006). Large-scale turnover of functional transcription factor binding sites in Drosophila. PLoS Comp Biol, 2006. 2:10. https://doi.org/10.1371/journal.pcbi.0020130 

Pollard DA, Moses AM, Iyer VN, Eisen MB (2006). Detecting the Limits of Regulatory Element Conservation and Divergence Estimation Using Pairwise and Multiple Alignments. BMC Bioinformatics, 2006. 7:376. https://doi.org/10.1186/1471-2105-7-376

 

M.S. Theses

Thuet-Davenport, Tanner, “Mapping Genetic Variants Associated with Dynamic Protein Abundance in Haploid Yeast” (2021). WWU Graduate School Collection. 1007. https://cedar.wwu.edu/wwuet/1007

Wienecke, Anastacia, “Codon bias and mRNA folding stability: Two natural controls of protein expression dynamics” (2020). WWU Graduate School Collection. 978. https://cedar.wwu.edu/wwuet/978Wienecke, Anastacia, “Codon bias and mRNA folding stability: Two natural controls of protein expression dynamics” (2020). WWU Graduate School Collection. 978. https://cedar.wwu.edu/wwuet/978

Conferences

Bold = Primary Presenter

2023

WWU Scholar’s Week

  • Talk (Three-Minute-Thesis) – Benjamin Haagen
  • Talk (Three-Minute-Thesis) – Minh Phan
  • Poster – Characterizing dynamic protein expression variation between haploid S. cerevisiae strains using RNA-Sequencing and Mass-Spectrometry. | Benjamin Haagen, Katie Lane, Noah Haight, Jasmine Chen, Dr. Daniel Pollard
  • Poster – Fine Mapping Causal Polymorphisms Underlying Differential Protein Expression in S. cerevisiae. | Minh Trang Phan, Jasmine Chen, Winter Yi, Maggie Barry, Dan Pollard
  • Poster – Investigating age-related loss of chromatin silencing in natural isolates of S. cerevisiae. | Olivia Dong, Aidan Corbin

EVO-WIBO

  • Talk – Natural variation in codon bias and mRNA folding strength interact synergistically to modify protein expression in Saccharomyces cerevisiae. | Dan Pollard
  • Talk – Mapping heritable genetic variation underlying protein expression dynamics during mating pheromone response in haploid Saccharomyces cerevisiae | Benjamin Haagen
  • Poster – Fine-mapping causal polymorphisms underlying differential protein expression in S. cerevisiae. | Minh Phan
  • Poster – Investigating age-related loss of chromatin silencing in natural isolates of S. cerevisiae. | Olivia Dong, Aidan Corbin

2022

WWU Scholar’s Week

  • Poster – Investigating Age-Related Loss of Chromatin Silencing in Saccharomyces cerevisiae. | Trinity Wilson, Aidan Corbin, Dan Pollard.
  • Poster – Local and distant polymorphisms acting on protein expression show gene-by-gene, and gene-by-environment interactions. | Nadine Tietz, Maggie Barry, Aidan Corbin, Daniel Pollard.
  • Poster – Finding genes with cis-acting mutations in S. cerevisiae. | Katie Lane, Tara Wirsching, Daniel Pollard.

2021

WWU Scholar’s Week

  • Poster – Mapping QTLs Underlying Variation in Dynamic FIG1 Protein Expression. | Tanner Thuet-Davenport, Benjamin Haagen, Erik Ehlers, Rebecca Yarbrough, Sam Herr, Gaea Turman, Garrett Strawn, Krista Miller, Amy Williams, Dan Pollard.
  • Poster – Nature vs Nurture: estimating the contribution of natural genetic variation on protein expression phenotypes in haploid S. cerevisiae. | Erik Ehlers, Benjamin Haagen, Sam Herr, Garrett Strawn, Tanner Thuet-Davenport, Rebecca Yarbrough, Krista Miller, Amy Williams, Nadine Tietz, Trinity Wilson, Maggie Barry, Jasmine Chen, Daniel Pollard.
  • Poster – Unpacking networks of trans-acting protein expression regulators during mating pheromone response in haploid S. cerevisiae. | Benjamin Haagen, Sam Herr, Erik Ehlers, Garrett Strawn, Tanner Thuet-Davenport, Rebecca Yarbrough, Krista Miller, Amy Williams, Nadine Tietz, Trinity Wilson, Maggie Barry, Jasmine Chen, Daniel Pollard

ASN/SSB/SSE Virtual Evolution Conference

  •  Talk – Mapping networks of heritable genetic variation acting on protein expression dynamics during mating pheromone response in haploid Saccharomyces cerevisiae. | Benjamin Haagen, Tanner Thuet-Davenport, Garrett Strawn, Erik Ehlers, Rebecca Yarbrough, Krista Miller, Amy Williams, Daniel Pollard.

2020

ASCB/EMBO Cell Biology Conference

  •  Poster -Mapping QTLs Underlying Variation in Dynamic Protein Expression | Tanner Thuet-Davenport, Benjamin Haagen, Erik Ehlers, Rebecca Yarbrough, Sam Herr, Gaea Turman, Garrett Strawn, Krista Miller, Amy Williams, Dan Pollard.