Mussel Management Mastery

June 5th, 2020

Today was the beginning of our long transit to the Florida Keys where we will be picking up the other PI (Principal Investigator) for the cruise, Dr. Craig Young from the University of Oregon. This transit is 57 hours meaning no Jason or Sentry dives for the next two days and the science party can catch up on science and sleep. Because of this, many of our team slept in today, and had a more casual approach to the workday after finishing Sentry sorting early in the morning. However, we did have a hefty list of science tasks to be done: sort tube traps, measure and dissect mussels, create highlight video clips, process carbonate rock for sponges, sort Tuffys, and animal maintenance.

As Blake Ridge was one of our deeper sites, 2160 meters, we had a new species of mussel to work with, Bathymodiolus heckerae. The three types of mussels we are collecting and processing on this cruise are Gigantitus childressi, Bathymodiolus brooksi, and Bathymodiolus heckerae. Each of these mussels are found at different depths: 400-2200 meters, 1080 – 3300 meters, and 2200-3300 meters respectively. In processing these mussels, first shell measurements of length, width, and height are taken for each individual. The shells are given to Ian Grace of NC State for geochemical analysis, and the body tissue is preserved in formalin for reproductive studies. Seen here is Caitlin Plowman (UO) dissecting B. heckerae in our dissection lab on board.

Meet a Scientist on Board

Hi everyone. My name is Caitlin Plowman. I am a PhD Candidate with Dr. Craig Young at the University of Oregon’s marine lab, the Oregon Institute of Marine Biology. I also did my bachelors and masters degrees at UO. My research focuses on the reproduction of invertebrates (animals without back bones) that live in the deep-sea. I have been fortunate enough to be on many research expeditions and absolutely love being at sea! On this research cruise, I am collecting mussel samples for my dissertation work and helping to ensure samples for our lab’s portion of the grant are collected and properly preserved. So far, I have earned the nicknames Chemical Queen because I prep and use most of our fixatives, and Mussel Serial Killer since I ask for mussel collections and then dissect them all.

Specimen Storage Showcase

With all of our samples coming from the deep sea, I’m sure everyone is curious about how we keep everything alive. The temperature difference is critical, as the deep sea is much colder than the surface. In order to combat this change in environment, we have two cold rooms on board. For shallower sites, we have one cold room at 8 degrees Celsius, and for deeper sites like Blake Ridge, we have another cold room at 4 degree Celsius. These cold rooms are where we store everything that comes up from Sentry and Jason while we work on separate tasks to ensure everything stays alive in the meantime. Each bucket and cooler filled with organisms is also fed an airline with an air stone that continually pumps oxygen into the containers. The other notable features in these rooms are having Bunjees to secure our smaller samples on the shelves, and a non-slip mat incase of spills or slippage from the moving vessel.

Fiona McBride (WWU) leads us into the cold room.
Mitch Hebner (WWU) works on animal maintenance within the cold room.

Honey I Shrunk the Cups

June 4th, 2021

What goes down must come up. Today began around 9 am when Jason returned from Blake Ridge with our collected samples. We recovered our sipunculan trap, tube traps, and tilt meter from last year’s cruise and deployed new traps that we’ll be recovering in the fall. Along with our recovered science instruments, our cups have returned! First, we completed our immediate tasks like emptying and siphoning the bioboxes, retrieving the niskin bottles that collect water samples, and putting specimens in the cold room. Then, we were able to see our shrunken artwork. As one of our deeper sites, we were all excited to send down these Styrofoam cups as they would shrink the most. To our pleasure, the cups were about half the size we sent them down! Even though we put paper in them before sending them down to ensure they wouldn’t fall into each other or shrink wonky, we had an amalgamation of resulting shapes. We had fun comparing cup structures and how our designs changed.

Pink Bathymodiolus sp. larvae.

With the samples back on board, the scientists get back on the scopes. We had multiple bins of sieved biobox water to search through. With these boxes carrying carbonate rocks, mussels, and our science traps, it’s important to sort through the sieved samples to find any hiding larvae. The countless hours we spend searching through sediment under the microscopes is like a long game of those iSpy books, searching for hidden targets. In these samples we are particularly searching for gastropod and bivalve veligers and are especially excited when we find the pink Bathymodiolus larvae pictured here. The scientists erupt into cheer and we all take turns seeing them under the lucky scope. With other similar shapes or colors like foraminifera and ostracods in our samples, we get really psyched for successful findings.

Meet a Scientist on Board

Howdy, my name is Leo Zaklikowski, I grew up in Tiburon, California and went to the Oregon Institute of Marine Biology, where I graduated with a degree in marine biology in 2018. After graduating I did a study abroad program in Thailand and when I returned, I worked as a fisheries observer out of Coos Bay and Newport, Oregon. This is my first time being on a research cruise, but my fiancée was on the R/V Endeavor cruise in the fall and inspired me to experience science at sea. I love polychaete worms as they are my friends, and I am truly a rainbow four leaf clover in the coal mine.

Tessa Beaver (WWU) and Fiona McBride (WWU) pose with the CTD outside on the deck.

Another way we are collecting data on this cruise is by doing a CTD cast at each location and depth where we deploy Sentry. CTD stands for conductivity, temperature, and depth, and is a tool that can help us understand the environment where we are finding larvae. The CTD (pictured to the left) is cast off the side of the boat and lowered on a winch to about 10 meters above bottom. On the way down, it takes a profile of the temperature, salinity, and several other parameters that allow us to choose interesting spots to take water samples from. On the way up, a scientist in the main lab is calling out various depths to the winch operator and firing the niskin bottles to collect discrete water samples.

Before we leave our site at Blake Ridge, we deployed Sentry one more time, with another late retrieval. Looks like the science team has another late night of sorting ahead of them!

From Up on the Bridge to Down on the Ridge

June 3rd, 2021

Our transit south has brought us to Blake Ridge, a deep site off the coast of South Carolina, where we arrived around ten in the morning after a 16 hour transit through the night. Shortly following our arrival, the crew and Sentry team coordinated a Sentry splashdown, and the autonomous submersible set off on a twelve hour quest to collect plankton samples above the site. We the science team had finished a majority of our sample processing from previous dives so we had time to relax and tie up loose ends. The Young Lab PhD students have the lengthiest sample processing, which involves among other things dissecting and preserving mussels from the seafloor.

Meet a Scientist on Board

Hello! My name is Fiona, I am an undergraduate REU student at Western Washington University. Though I have spent time on boats before (sailboats are my favorite, from college racing dinghies to hundred-foot schooners) this is my first research cruise and my biggest boat. This cruise has been long-awaited, as I actually applied for the REU (Research Experience for Undergraduates) over a year ago, before covid came and brought travel to a halt. Now, even with masks and quarantine, I am very happy to have the unique experience of being aboard. Back home in Washington I study snow algae in the Kodner lab at WWU. I enjoy good hikes, animals of all shapes and sizes, art, and gardening.

During this cruise I normally spend slower days lounging on deck as much as possible – standing outside long enough, you can often spot dolphins or, now that we’re in warmer weather, flying fish – but the past few days have been extremely humid. Also, I had my own work to do. As an undergraduate at Western Washington University, I am still taking a couple of classes while onboard. Our data allowance (400 mb/day) has thankfully been enough to let me keep up with recorded lectures and assignments since my classes are still online. I was able to kick back in the library to do some readings and take a final exam for Developmental Biology of Plants. The quarter is wrapping up, and I am thankful that the transit to our next site will be long enough to give me time to finish our homework.

The crew also treated us today with a tour of the bridge. The Thomas G Thompson has a high tech navigational system, a feature that is particularly helpful on a research ship. In addition to standard shipboard equipment like  sonar and AIS, the ship contains an autopilot system which can be used to automatically hold position on precise coordinates while Jason, Sentry, or the CTD casts are in operation. While on the bridge we were also encouraged to take turns sitting in the captain’s chair and look out on the ocean, to receive the full experience, we were also granted some binoculars! Like the science team has been doing, there are also watch rotations among the captain, mates, and crew to make sure everything is running smoothly. The bridge tour really made us appreciate all the technology, resources, and most importantly the people that make this research possible. It also provided insight as to why it’s so noisy in our sleeping quarters when we are at a site. The bow thrusters that maintain our position are working hard, but are right next to where we sleep.

Dexter Davis (WWU) observes from the captain’s chair.
Left to right: Sean (Sentry), Tessa (WWU), Casey (UO), and Lauren (UO) retrieve Sentry samples.

Later in the day the science team had a late night. Sentry resurfaced around 23:00 (11 pm) and as we are searching for larvae, we have to sort the samples as soon as possible. This means we don’t sleep until we’re done! Pictured here is the teamwork between Tessa Beaver (WWU), Sean (Sentry), Casey Barnard (UO) and Lauren Rice (UO) lifting and rinsing down the “plankzookas” of Sentry’s SyPRID to retrieve the plankton samples. Once the collection tank is retrieved and poured into a bucket, the samples are whisked away for processing.

With Sentry safely on board the RV Thompson, Jason gets ready to dive down to the site. Typically Jason and Sentry are not in the water at the same time to avoid entanglement with Jason’s cable tether to the ship and ensures no collisions are possible. These are expensive machines and need to stay in prime condition to ensure all our science goals can be met. Tomorrow we will work with Jason samples and redeploy Sentry for one more collection before leaving Blake Ridge.

Scanning the Seafloor, Smelling the Seafloor, Sketching the Seafloor

June 2nd, 2021

Today began with a continuation of the Jason dive that began yesterday. After 21 hours searching for the lost mooring on the ocean floor, we were unable to locate where it ended up. Being in the Gulf Stream, the current was quite strong and could have influenced the movement of our target. To search for it, we did parallel transects 20 meters apart surrounding our “best guess” location. Eventually we had to give up the search and start transiting to our next site: Blake Ridge. Unfortunate we weren’t able to find the mooring, but we did our best and have to continue with the main goals of the cruise. I (Liam) had my first and second 4-8 (AM and PM) shifts in the Jason van, where I spent my time logging events onto SeaLogger. While it was a great experience, it was rather boring and included lots of the same thing: ocean floor and blue water. Nevertheless, it was awesome to see how they operated Jason first-hand! Giving the undergraduates a chance to work shifts in Jason was amazing and felt like a science fiction movie with the amount of screens and data coming in regularly.

Liam Patrick (CSUMB) and Casey Barnard (UO) work their shift in the Jason van.

While Jason was attempting to recover the mooring, the science team took the time to sort through our collected tube traps from the two previous sites. These tubes were packed with Dimethyl Sulfoxide (DMSO) and were placed to collect larvae from our seep sites. When opened, these samples were full of amphipods that had been sitting at the bottom of the ocean for over a year. They did not smell good. Sharing our lab with the Sentry team, we received many hilarious comments about the stench. Think mussel septic tank fermenting for a year. Luckily wearing face masks for COVID safety doubled as a stench blocker! We sorted through the 4 tube traps relatively quickly however, and even managed to find one of our target species, a Bathymodiolus larval shell!

Meet a Scientist on Board

Hi all! My name is Liam Patrick. I am an undergraduate student from California State University Monterey Bay joining WWU/the Arellano lab as an REU (Research Experience for Undergraduates) student. I have one more semester left at CSUMB, super close to majoring with a B.S. in Marine Science. After that, I have no idea!

I was born in Scottsdale, AZ, but raised in Anacortes, WA (also home to WWU’s Shannon Point Marine Center). In my free time, I am an avid beachcomber, dinghy sailor, and sailing instructor. I enjoy SCUBA diving as much as possible and I’m planning on being certified for advanced SCUBA through my college this next semester (super excited). Other than that, I spend my time hiking or playing video games with friends, especially during quarantine!

The opportunity to join this cruise rose out of the WWU REU program at SPMC. While I know almost nothing about larvae, I saw the cruise as an amazing opportunity to learn and experience life on a research cruise. Currently, I am assisting others when I can and taking part in various graduate projects. Once we start into the Gulf of Mexico, I will be focusing more on helping Mitch’s project with T. Naticoidea larvae, a deep-sea snail that can be found around hydrocarbon seeps at depths from 400-1700 meters. By helping with that, I hope to hone into my own research question concerning these larvae or something similar for my REU.

Later in the day, in preparation for one of our deeper sites, Blake Ridge – 2160 meters deep, the science team and anyone else who felt inclined, made cups! This is a staple on any deep sea research cruise. Styrofoam cups are drawn on with every color of Sharpie that shrink when brought to the ocean floor and back. The cups are placed in a mesh bag and attached to Jason, where the increasing pressure causes the cups to compress, and when returned to the surface retain the artwork on a miniature cup. We shared stories about who we are making cups for and what the artwork represents, had a lot of laughs, and became closer as a group. We also discovered there are quite a few artists in our group! Some of our cups boasted familiar characters like our submersibles Sentry and Jason, others highlighted marine animals and our seep sites.  Now we are all eager for the Blake Ridge dive to see how they all turn out! Here’s a pictured of our cups displayed, each one so unique!

Current Events

June 1st, 2021

Today the science party is getting a much-deserved break from the hustle and bustle of 24-hour operations with both AUV Sentry and ROV Jason! After arriving on site late last night, ROV Jason was deployed in hopes of recovering a lost oceanographic monitoring mooring that failed to surface several months ago. The first  mooring was successfully recovered and brought on deck early this morning. Unfortunately, we also brought up a shark that had been laying on the mooring. Here’s a picture of how we found it, a shark hotspot! Now, we are transiting to another site to try and recover a second mooring that also failed to surface. It is unclear whether we will be able to find it, as several storm systems have come through this area and may have buried it under meters of sediment. Fingers crossed that we could find and recover it later tonight!

In other news, the ship has finally entered the Gulf Stream and the water is blue-er than many of us have ever seen. There is lots of free-floating Sargassum (a type of seaweed) with plenty of tiny critters that are likely hitching a ride on the surface currents. Not to mention, the weather is treating us very well! After a day spent finishing up science objectives from the most recent site, you can find us lounging in the sun, reading books, and enjoying ship life … our group is quite content.

Sharks on PEACH mooring.
Mooring retrieved!

Meet a Scientist on Board

Hi I’m Tess! I’m a master’s student in Dr. Shawn Arellano’s deep sea larval lab. I grew up in Olympia, WA, and fell in love with the ocean as a little kid on my family’s boat exploring the Salish Sea. After completing an Associate’s of Biology at Pierce College, I transferred to Western Washington University and got a Bachelor’s in Biology with Marine Emphasis. I did various projects as an undergrad, ranging from the reproductive ecology of nudibranchs to maintaining long-term water quality datasets in freshwater systems. When I graduated, I was given the opportunity to begin my Master’s in Dr. Arellano’s lab studying critters of the deep and their planktonic babies. Since then, I have been able to explore some of my passions in bioinformatics and DNA sequence analysis and have built a project around those skills. I am studying how the microbial composition of the cold-seep mussel, Gigantidas childressi, changes during key life-history transitions in both diet and habitat.

            This is my third time going to sea for science! My first research cruise was in March of 2020 on the R/V Atlantis working with DSV Alvin (a human operated submersible!), a second cruise in October-November 2020 was focused on collecting deep-sea larvae with AUV Sentry. I am especially excited for this cruise because I will be collecting many of the samples needed for my thesis with ROV Jason and Sentry.

Jason has now been in the water since 3:30PM, and because of the strong current in the Gulf Stream, on our 2800m descent, we drifted 5km from our target “best guess” of where the mooring is. It will take us a couple hours to fight this current and make our way over to where we initially meant to recover the mooring. This means we have a long night ahead of us, as Jason shifts are 24 hours around the clock, with 4-hour shifts taken on by the undergraduates currently. This will put to the test the diligence of our team and test our abilities to sleep on our off time but be ready for our next shift. Let’s hope we can find the mooring in time before we begin our transit tomorrow to Blake Ridge!

Sentry Flies, Dolphin Eyes, Time to Mobilize

May 31st, 2021

Happy days! The weather cleared up enough for us to deploy Sentry today. The chiclet with bazookas was successfully deployed and we performed a short dive to quickly get a sample and be on our way. Once the sample was back on board, scientists eagerly rushed back to the scopes to explore the mysterious microworld just outside our portholes. Bowls swirled, scopes zoomed in an out, short dance parties were held, ice trays were filled, and larvae were sorted. All of this to assess different morphotypes of larvae to understand larval distribution within the water column. We finished this collection relatively quick, pleased with the lack of shrimp in these samples. We found a crab zoea, a shrimp zoea, a doliolarian, and some nectochaetes.

After sorting, the scientists got a well-deserved break with a 13-hour transit to the next site. Before our next science stop at Blake Ridge we are recovering some oceanographic moorings. These moorings are part of the PEACH Project under multiple institutions that collect data for storm analysis using ADCPs (Acoustic Doppler Current Profilers). Some of these moorings were lost at sea, they were supposed to surface on their own, and now we are retrieving them from the bottom of the ocean. There are two moorings at separate sites on our way, so we will spend tomorrow trying to find them and get them on board.

Meet a Scientist on Board

Hi, my name is Dexter Davis, and I’m a senior undergraduate from Western Washington University studying marine science and minoring in mathematics. I am also the one that has been writing these blog posts! I work for Dr. Shawn Arellano as an undergraduate research assistant and I’m incredibly excited to be back at sea. I was privileged to be on the RV Atlantis cruise with Alvin last spring, and looking forward to working with Sentry and Jason on this one. I’ll be graduating on June 12 while at sea, which I think is a spectacular way to end my undergraduate career. Post-graduation I’m hoping to work as a research assistant or a lab technician, to explore and hone my interests in marine science before returning to school for a master’s degree. I currently have interests in deep sea research and exploration, fisheries science, climate science, as well as species interactions. The ocean is so vast and full of things to discover, it creates a career full of excitement and unique experiences and I’m excited to get out into the marine science world.

On our day off the scientists spent the day scanning the horizon from the bow of the ship and had a late movie night. In hopes of seeing whales and dolphins, the scientists regularly perform the “cetacean dance”, beckoning the charismatic mammals to visit our ship. Sometimes there’s a lag and they don’t come immediately, but this time it worked quite well! Not only did we pass through a large pod of pilot whales, estimating around 100 individuals, but we also encountered a superpod of dolphins! These curious and playful dolphins swim right under the bow to play in the waves, and you could watch them seeking out the rest of the pod to join in on the fun. After the dolphins had left and the sun had set, the undergraduates had a movie night in the lounge on board. This room is full of all the movies and TV shows you could think of, with all genres for any taste. Candy was had, and the ice cream fridge was raided.

Tomorrow begins an exciting day for the undergraduates, will all of them taking over Jason shifts during the mooring recoveries. They will be on board to record and report on the progress of these lost instruments in an attempt to reunite them with their owners. A deep sea search party with some seafloor exploration is quite the experience to have under one’s belt! Very excited to see what awaits us!          

Whether We Wanted to or Not, We Had to Wait on the Weather

May 30th, 2021

Hoping to sleep through rough waves, we awoke to another stormy day and learned a new term: W.O.W, Waiting on Weather. As we have our submersibles on board, when the boat is rocking too much, we want to wait so there’s no extra strain on gear or potential for collisions with the vessel. Because of this, we were unable to launch Sentry today to get plankton samples and will try again in the morning.

Some of us were unbothered and continued working, while others could only stomach short periods of time under the microscope or even just being awake. Everything becomes more difficult as the waves slosh; your balance is lost as you traverse the corridors, anxiety of falling in the shower increases (there’s actually a bar in the shower to hold on to!), keeping the microscope focused becomes harder, and you become disorientated. This is life at sea, and observing the horizon going above and below the boat won’t be a one-time experience, but a surreal one. 

The boat goes up.
The boat goes down.

Meet a Scientist on Board

Hi, my name is Laura Murray and I’m a graduate student from Western Washington University. I received my bachelor’s in science studying microbiology at California Polytechnic State University in San Luis Obispo. I am continuing my microbiology degree under Dr. Craig Moyer studying the microbiology of hydrothermal vent chimneys, where little microbes live in the distinctive pillars at vent sites. I was  inspired to pursue microbiology after watching the movie Contagion and see how microbes affect our lives. I moved to Washington after my sister enrolled at Western and I perused the graduate programs.

Science Behind the Scenes

Scientist measures mussels with calipers in the ship's lab.
Sinja measures many mussels

What are we doing with the mussels we are collecting from each site? When the days slow down, one thing we do is measure the mussels. Once consolidated, we measure the juveniles and adults with calipers to quantify size distributions. Pictured here is science teamwork at its finest; Sinja with the calipers, and Avery as the scribe, efficiently working through buckets full of bivalves.

We hope tomorrow’s weather clears up enough to get Sentry back in the water. It’s crucial at every site to deploy Sentry and collect water samples at multiple depths using the SyPRID larvae collectors. Exploring whether or not our target larvae species are within the water column at our sites is pivotal to answering out questions surrounding cold seep larval dispersal.

Of Lightning and Larvae

May 29th, 2021 – post by Casey Barnard (UO) and Fiona McBride (WWU)

Today we arrived at our next site Chincoteague around midnight on Saturday. Soon after arrival, ROV Jason was deployed. This site is around 1km in depth and takes around an hour for Jason to descend and another hour to ascend. The total amount of time Jason spent in the water was 12 hours. Pictured here are the boxes of goodies Jason brought back to the surface at 04:30 PM. If you look closely you can find a box filled with mussels and a box filled with larval traps! These “bio boxes” have Bungee cords that keep them closed during descent and ascent to make sure we don’t lose anything. The Jason divers are experts in using the robotic arms to make the smallest movements including stretching these cords over the boxes. In the evening a CTD was deployed for water samples at various depths.

In the meantime, the weather has not been in our favor since arriving here, with swells up to 12ft rocking the boat and lighting all around us. Those who could stand the rocking helped sort larvae, measure mussels, and filter water from the Baltimore Canyon site. We finished our processing around the turn of the day and most of us went to bed to wait out the storm and process Chincoteague’s samples the coming morning. It’s best to recover and conduct science when we’re at 100%.

Meet a Scientist On Board

Hi my name is Casey Barnard and I am a senior undergraduate from the University of Oregon helping out the folks in Craig Young’s lab. I will be graduating while onboard on June 14th with a Bachelor of Science degree in Marine Biology. This summer I will be the TA for the Oregon Institute of Marine Biology’s Invertebrate Zoology course. And then shortly after summer ends, I will be moving across the country for graduate school at the University of North Carolina Wilmington starting my master’s degree in Coastal and Ocean Management.

I have spent most of my time aboard looking into microscopes and helping out wherever is needed. This is my first cruise, and it was going pretty well until we hit these rougher waters around Chincoteague. My biggest accomplishment so far was when I sat at a microscope sorting larva for around 16 hours straight (with some food breaks). Everyone onboard the TGT including the scientists, the Sentry Crew, the Jason Crew, and the ship crew are all so friendly that even when the weather is bad moral stays high!

 

Animals not under the microscope

The ship’s passenger list is growing! We now have at least five stow-away birds sheltering onboard the Thompson. They most likely were on the ship before we left port, but now they’re along for the ride! Though we enjoy their company we don’t necessarily want them in our lab space. Pictured here is one of two instances (so far) in which Avery has caught birds belowdeck and brought them outside. We’ve started naming our friends and this is Gertrude the Wanderer. 

Now we’ve got a busy day ahead of us, with the second dive of our Atlantic sites completed, and what feels like we’ve only just completed the Baltimore Canyon samples, it’s back to the microscopes! These long hours are crucial to finding the larvae hidden in the sediment and we have to sort through every last drop (literally!). Hoping for calmer seas tomorrow so we can concentrate harder on finding larvae instead of not being sick!

Jason Dives and Science Thrives

May 28th, 2021

At 12:00am, Jason managed to finally get into the water and began a 12-hour dive at Baltimore Canyon. Since the vehicle is remotely operated, longer dives are possible, and science is done on 4-hour shifts. There’s a 12-4 shift done by Caitlin, Tessa, and Ian, a 4-8 shift by Sinja, Lauren, and Avery, and a 8-12 shift by Shawn, Dexter, and Mitch, both AM and PM shifts. This ensures science goals can be met around the clock efficiently with no one getting too tired or hungry. The pilots also exchange during shifts. 

Seen in this picture above, Jason is lifted off the boat by a winch that lowers it into the water, and keeps a cable connected for control. This cable has buoys attached to it as Jason descends, to ensure the cable floats above Jason and does not tangle while Jason is moving. There is also a sensor attached on the cable that communicates with the control room when to reel in or let out the winch and allows the ship to move back and forth without tugging on Jason.  

Liam Patrick (Left), Dexter Davis (Middle), and Mitch Hebner (Right) sieving the bio boxes.

Once Jason has completed the dive, the scientists get ready to bring in the recovered science equipment, collected mussels and carbonate rock. We have to act quickly before anything is lost due to the changing water and temperature from coming to the surface. We have teams for the various tasks that need to be done to ensure everything is done in a timely manner and before another dive is completed.

Seen here is the slurp and sieving team. During the dive, our recovered science is put into “bio boxes” in the basket on Jason. This team siphons out all of the residual water and sediment from these boxes, as well as the “slurp” samples collected by Jason’s vacuum hose, and sieves it down to 250 microns to catch any larvae found at the site. Some scientists react differently when asked for a photo op! With the results of the sieve, this sediment will be sorted through under the microscope to locate the larvae of target species, notably the pink shelled mussels.

Meet a Scientist on Board

Hi! My name is Carmen Sanchez-Reddick, and I am an undergraduate marine biology student and member of the Young lab at the Oregon Institute of Marine Biology. Usually, you can find me counting oocytes in tunicate specimens for my undergraduate thesis, making breakfast for the residents of the Charleston Marine Life Center, or working on homework in the library. This is my first ever, but hopefully not last, research cruise, and it has been an incredible experience thus far. Watching Sentry and Jason deployments, laughing with new friends, and eating delicious food have become constants in my day-to-day routine and have kept me going during bouts of sea sickness. I’ve spent most of my time sorting larvae, and I hope to be able to distinguish larvae, egg casing, and dirt by the end of the cruise!

With the first Jason dive of the cruise, the scientists have their hands full! The delay in blog posts has been due to the hard-work of everyone on board. In addition to sorting through the sieved sediments, adult mussels are to be dissected, juveniles measured, sipunculan traps opened and counted, animals need to be organized and put into the cold room and given proper housing, carbonate rock needs to be assessed for sponges, among many other tasks. As we continue through this cruise we will explain all these tasks and what purpose they serve in answering our research questions, stay tuned! 

Technical Difficulties at Sea

Sentry, the autonomous submersible used to conduct plankton tows on this cruise. The two large black circles are funnels that lead into collection tubes.

May 27th, 2021

While staying busy sorting through Sentry samples for larval morphotypes, today we encountered unexpected delays in our science. Gearing up for our first Jason dive (Jason is our remote operated submersible on board) was cut short when problems arose with the winch that moves Jason from the ship into the water, and we can’t visit our site at Baltimore Canyon until the issue is found and resolved. Diving with Jason is essential for this project; retrieving scientific instruments from last year’s spring cruise, collecting new mussels and carbonate rocks, as well as deploying new scientific equipment all requires Jason and associated machinery to be functioning properly.

Data Collection Behind the Scenes

During this cruise we are attempting to collect many larvae and larval shells to answer the questions about cold seep larval dispersal. One way we are collecting these samples is through the use of these tubes traps! Seen here are Avery Calhoun,  Lauren Rice, and Caitlin Plowman (graduate and PhD students from UO) filling the falcon tubes in each arm of the trap with a formalin preservative.

How this trap works is by slowly releasing this chemical into the water above the trap, and any larvae swimming past will die and sink into the trap, collecting them all at the bottom of the tube until the trap is collected. These rubber straps stretched over the tubes are to ensure the traps do not lose the solution on the journey down to the site in Jason, with a galvanic release that will dissolve under 24 hours in saltwater subsequently allowing the solution to escape. Another interesting feature is the yellow monkey’s fist knot that is attached to the trap. This will float above the trap and will allow Jason’s arms to easily grab and move the trap around!

Meet a Scientist On Board

Hey everyone! My name is Michael Gray, and I am a student under Dr. Roy He at North Carolina State University. With this cruise, I will be completely a double major with a B.S. in Physics and a B.S. in Marine Science with a concentration in Physics. In the fall I’ll be continuing at NCSU in pursuit of an M.S. in Physical Oceanography.

Usually, I tend to focus on tracking Nor’easter storms and trying to determine what makes them tick, but this cruise has taken that on its head and spun it around quite a few times! While I do perform some physically oriented tasks such as processing data from tilt meter (devices that use their orientation in the water to determine the speed and direction of a current) and casting CTDs (measures conductivity, temperature, and depth), I have been taken to the extremes of my comfort zone to sorting larvae?!

I definitely have no idea what I’m doing, but I’d say I’m blending in well enough (not at all). Today, however, was a pretty slow day. We deployed Sentry around 12:30pm and another delay in Jason being deployed meant a lot of sitting around and waiting for Sentry to return with more samples to sort.

Hopefully the problem with Jason can be fixed and it can be deployed at least once per site, otherwise a lot of the projects being conducted by Grad and PhD students aboard will fall vastly short of target samples. Until then, we’ll just be hitting a lot of late-night sorting montages!

We hope that this issue will be found soon and we will be able to start collecting data from our site, 400 meters deep! With the brunt of our sampling coming from our sites, everyone is antsy to get in the water, but also so we can move to our next site.

Science team gets familiar with the ROV Jason